GST pull-down assay with substituted forms of human ERH.
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posted on 2013-08-28, 01:46 authored by Monika I. Banko, Marek K. Krzyzanowski, Paulina Turcza, Zuzanna Maniecka, Marta Kulis, Piotr KozlowskiIndicated FLAG-tagged ERH forms incubated with either GST-tagged fragment L7 of human PDIP46/SKAR (GST-PDIP46/SKAR[L7]) or GST-tagged fragment B of human Ciz1 (GST-Ciz1[B]) and detected by western blotting with anti-FLAG antibody followed by enhanced chemiluminescence reaction. PDIP46/SKAR does not interact with ERH H3A Q9A or ERH H3A Q9A E37A T51A, and Ciz1 does not interact with ERH E37A T51A or ERH H3A Q9A E37A T51A.
