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GLUT3 is abundant in macrophages isolated from human atherosclerotic plaques and in hypoxic human macrophages.

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posted on 2013-02-20, 01:24 authored by Lu Li, Bo Liu, Liliana Håversen, Emma Lu, Lisa U. Magnusson, Marcus Ståhlman, Jan Borén, Göran Bergström, Malin C. Levin, Lillemor Mattsson Hultén

(A) Representative immunohistochemical staining of sections of a human atherosclerotic carotid plaque with antibodies against GLUT3, HIF-1á and the macrophage marker CD68. (B) GLUT3 and HIF-1á mRNA expression in CD14+ macrophages isolated from 8 human atherosclerotic carotid plaques. (CF) GLUT3 mRNA and protein levels in human monocyte-derived macrophages cultured in medium containing 11 mmol/l glucose for 24 h in normoxia (21% O2) or hypoxia (1% O2). (C) Real-time RT-PCR analyses of GLUT3 mRNA expression. Data are mean ± SEM from 6 macrophage donors. (D) Representative immunoblots of GLUT3 and tubulin in normoxic (N) and hypoxic (H) human macrophages. (E) Quantification of immunoblots. Data are mean ± SEM from 6 macrophage donors. (F) Representative immunoflourescent images of macrophages cultured in normoxia or hypoxia and stained for GLUT3 (green) and nuclei (blue). Scale bar 10 µm. **P<0.01, ***P<0.001 vs normoxia.

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