GLUT3 is abundant in macrophages isolated from human atherosclerotic plaques and in hypoxic human macrophages.

(A) Representative immunohistochemical staining of sections of a human atherosclerotic carotid plaque with antibodies against GLUT3, HIF-1á and the macrophage marker CD68. (B) GLUT3 and HIF-1á mRNA expression in CD14⁺ macrophages isolated from 8 human atherosclerotic carotid plaques. (C–F) GLUT3 mRNA and protein levels in human monocyte-derived macrophages cultured in medium containing 11 mmol/l glucose for 24 h in normoxia (21% O₂) or hypoxia (1% O₂). (C) Real-time RT-PCR analyses of GLUT3 mRNA expression. Data are mean ± SEM from 6 macrophage donors. (D) Representative immunoblots of GLUT3 and tubulin in normoxic (N) and hypoxic (H) human macrophages. (E) Quantification of immunoblots. Data are mean ± SEM from 6 macrophage donors. (F) Representative immunoflourescent images of macrophages cultured in normoxia or hypoxia and stained for GLUT3 (green) and nuclei (blue). Scale bar 10 µm. **P<0.01, ***P<0.001 vs normoxia.