GLIC current activation was slow and deactivation fast compared to GABA_ARs.

A) GLIC currents activated by a 2 s application of pH 4.0 buffer exhibited two different desensitization profiles. In 25 patches, little current desensitization was observed, whereas in 18 patches, a fast component of desensitization was observed. GLIC current activation and deactivation was analyzed separately for each group. Expanded views of current activation and deactivation are shown to the right and fit with bi-exponential functions (red curves). B) The τ_w-activations for GLIC currents (pH 4.0) from both groups are plotted and compared to the τ_w-activations of currents obtained from patches from mock-transfected cells (pH 4.0) and from GABA_ARs (10 mM GABA). Data are mean ± SEM (GLIC no early: n = 25, GLIC w/early: n = 18, Mock: n = 7). C) The τ_w-deactivations are plotted for both groups of GLIC currents following a 2 s (as shown above) as well as a 25 s pulse of pH 4.0 buffer. The τ_w-deactivations for GABA_AR currents evoked by 5 ms or 1 s applications of 10 mM GABA are also plotted. Data are mean ± SEM (GLIC no early 2 s: n = 11, GLIC no early 25 s: n = 8, GLIC w/early 2 s: n = 6, GLIC w/early 25 s: n = 6).