GBS adapters, PCR and sequencing primers.
figure
posted on 2011-05-04, 02:10 authored by Robert J. Elshire, Jeffrey C. Glaubitz, Qi Sun, Jesse A. Poland, Ken Kawamoto, Edward S. Buckler, Sharon E. Mitchell(a) Sequences of double-stranded barcode and common adapters. Adapters are shown ligated to ApeKI-cut genomic DNA. Positions of the barcode sequence and ApeKI overhangs are shown relative to the insert DNA; (b) Sequences of PCR primer 1 and paired end sequencing primer 1 (PE-1). Binding sites for flowcell oligonucleotide 1 and barcode adapter are indicated; (c) Sequences of PCR primer 2 and paired end sequencing primer 2 (PE-2). Binding sites for flowcell oligonucleotide 2 and common adapter are indicated.
