Fusarium culmorum wild types and ectopic transformants penetrate into wheat seeds and kill the seedling while ΔFcStuA mutants are unable to penetrate the seed.
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posted on 2013-02-23, 11:41 authored by Matias Pasquali, Francesca Spanu, Barbara Scherm, Virgilio Balmas, Lucien Hoffmann, Kim E. Hammond-Kosack, Marco Beyer, Quirico MigheliSeed viability after 3 days in the dark at 25°C on water agar (germination) after previous plug inoculation for 3 days with the two wild types (FcUK99 and Fc233B), the mutants (S12, S13 and S19), the ectopic transformants (S2 and S20) and a mock control inoculated with 10 µL water (W). Seeds were washed with NaClO before plating to eliminate external mycelium.
