Figure_1.tif (462.5 kB)
Flow chart for the preparation of microsomes from Sf21 cultured insect cells.
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posted on 2014-12-08, 03:10 authored by Toru Ezure, Kei Nanatani, Yoko Sato, Satomi Suzuki, Keishi Aizawa, Satoshi Souma, Masaaki Ito, Takahiro Hohsaka, Gunnar von Heijine, Toshihiko Utsumi, Keietsu Abe, Eiji Ando, Nobuyuki UozumiFewer centrifugation and wash steps were required compared to the conventional protocol for the preparation of rough microsomes from dog pancreas and the composition of the buffer was simplified. Single-tube reactions consisting of Sf21microsomes, cell-free translationally active lysates from cultured insect cells and mRNA were performed to synthesize membrane proteins in vitro.
