Flow chart for mutant development.
figure
posted on 2013-10-21, 03:39 authored by Jessica L. Linville, Miguel Rodriguez Jr., Miriam Land, Mustafa H. Syed, Nancy L. Engle, Timothy J. Tschaplinski, Jonathan R. Mielenz, Chris D. Cox(A) The flow chart shows the mutant development process from the initial WT strain through the final mutant. Single colony isolate 6 was chosen as the final 17.5% v/v Populus hydrolysate tolerant mutant strain (PM). (B) Initial growth of WT culture on various concentrations of Populus hydrolysate to determine inhibitory effect. The culture in 5% v/v Populus hydrolysate started growing late in the fermentation. (C) An example of serial transfers showing increased growth over time in 10% v/v Populus hydrolysate. Illustrative curves show each daily serial transfer.
