FOXO1 Binds to −125/−91 of the Fshb Promoter.

TnT Flag-FOXO1-CA was incubated with a consensus FBE, −305/−271, −275/−241, −245/−211, −215/−181, −185/−151, −155/−121, or −125/−91 Fshb probes and tested for complex formation in EMSA. FOXO1-CA-DNA complex on the FBE is shown in lane 1, IgG control in lane 2 and Flag supershift is shown in lane 3. The FOXO1-CA-DNA complex (arrow) and antibody supershift (ss) are indicated on the left and right of the gel.