Figure_7.tif (2.41 MB)

Expression patterns of xrep-1 cDNA::gfp fusion genes in the xrep-1(k1007) background.

figure

posted on 2010-06-17, 01:34 authored by Koichi Hasegawa, Johji Miwa

GFP represents XREP-1 expression and RFP indicates GST-4 expression, without (a, c, d, e) or with 500 mg/L of acrylamide (b, f). (a) Fluorescence image of MJCU086. xrep-1aProm acDNA::gfp completely rescues xrep-1(k1007) GST-4::RFP expression. (b) Fluorescence image of MJCU086. After acrylamide treatment xrep-1aProm acDNA::gfp induces GST-4::RFP expression normally. (c) Fluorescence image of MJCU088. xrep-1bProm bcDNA::gfp does not rescue k1007 GST-4::RFP expression. (d) Fluorescence image of MJCU097. xrep-1aProm bcDNA::gfp does not rescue k1007 GST-4::RFP expression. (e) Fluorescence image of MJCU091. xrep-1aProm acDNAEx4-11::gfp completely rescues GST-4::RFP expression. (f) Fluorescence image of MJCU091. After acrylamide treatment xrep-1aProm acDNAEx4-11::gfp induces GST-4::RFP expression normally. Anterior is left, scale bars 200 µm.