Expression of vimentin in 3D cultures of Dcn−/− fibroblasts treated with either decorin or decorin core (core) at day 6.
Immunofluorescence staining of vimentin (A), decorin (C) and collagen type I (D) in µ-slide VI, visualized by Alexa 488 conjugated secondary antibody (green). Nuclei were stained with DAPI (blue). Images show merged z-axis layers of the complete 3D matrix. B: Quantification of vimentin immunofluorescence signal in merged layers normalized to cell number (nuclei) per image. Data represent 5 independent experiments and are expressed as mean ± SD (for each condition and experiments, 15 images were evaluated; **, p<0.01). Bar = 100 µm.