Figure_3.tif (979.9 kB)

Expression of sasC or sasC subfragments.

figure

posted on 2013-02-21, 04:05 authored by Katrin Schroeder, Mario Jularic, Samantha M. Horsburgh, Nina Hirschhausen, Claudia Neumann, Anne Bertling, Anja Schulte, Simon Foster, Beate E. Kehrel, Georg Peters, Christine Heilmann

A: SDS-PAGE (10% separation gel) of cell lysates of S. carnosus or S. aureus expressing sasC or sasC subfragments. The lanes contained: 1, 10, 15, marker proteins; 2, S. carnosus (pCX19); 3, S. carnosus (pSasC4074); 4, S. carnosus (pSasCsub1); 5, S. carnosus (pSasCsub2); 6, S. aureus SH1000; 7, S. aureus SH1000 (pSasC4074); 8, S. aureus SH1000 (pSasCsub1); 9, S. aureus SH1000 (pSasCsub2); 11, S. aureus 4074; 12, S. aureus 4074 (pSasC4074); 13, S. aureus 4074 (pSasCsub1); 14, S. aureus 4074 (pSasCsub2). The sizes of marker proteins are shown on the left (prestained protein ladder; Fermentas, Leon-Rot, Germany); in the right margin the sizes (kilodaltons) of SasC and truncated SasC proteins are indicated. B: Expression and purification of 6 x His-DUF1542. SDS-PAGE (10% separation gel) of crude cell lysates from non-induced E. coli (pHis-DUF1542) (lane 2), IPTG-induced E. coli (pHis-DUF1542) (lane 3), and purified 6 x His-DUF1542 (1.5 µg) (lane 4). The size of one marker protein is shown on the left (lane 1; prestained protein ladder, Fermentas).