Expression of oligodendrocyte-specific cell type markers in F3.Olig2 cells and expression of motor neuron-specific cell type markers in F3.Olig2-Shh cells.
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posted on 2014-05-20, 02:52 authored by Hong J. Lee, Kwang S. Kim, Jin Ahn, Hye M. Bae, Inja Lim, Seung U. KimF3.Olig2 cells are immuno-positive for oligodendrocyte markers CNPase (A) and galactocerebroside (GalC) (E), but F3.Olig2-Shh cells are negative for both oligodendrocyte markers (I and M). F3.Olig2-Shh cells are positive for motor neuron specific markers, ChAT (J) and Hb9 (N), but F3.Olig2-Shh cells are negative for both oligodendrocyte markers (B and F). These results indicate that F3.Olig2-Shh cells are bona fide motor neurons. The bar indicates 20 µm.
