Expression of Ret and its signalling partners in adult thymic populations.

A. DN, DP, SPCD8⁺ and SPCD4⁺ thymocytes were purified by flow cytometry. Results show quantitative RT-PCR normalized to Hprt1. Error bars show s.e.. Results from three independent measurements are represented. B. RET expression in DN and DP thymocytes was determined by flow cytometry. RET: black bold line; Isotype control: grey line. C. Thymic DN, DP, SPCD8⁺ and SPCD4⁺ thymocytes and CD45⁻ cells were purified by flow cytometry. Quantitative RT-PCR analysis was normalized to Hprt1. Error bars show s.e.. Results from three independent measurements are represented. D. DN1–2 and DN3–4 thymocytes were purified by flow cytometry. Results show quantitative RT-PCR normalized to Hprt1. Error bars show s.e.. Results from three independent measurements are represented.