Expression of D2-GFP fusion protein in E. coli (DH5α) is driven by a cryptic promoter in the cDNA encoding the 5′ 1–170 nt of DENV2 RNA genome.

(A) Schematic for the constructs of pT7-D2-GFP, pD2-GFP and pT7-GFP. (B) Florescence microscopy images of E. coli cells transformed with these plasmids. (C) Western blot analyses of transformed E. coli lysates employing 6F3.1 anti-dengue 2 virus core protein monoclonal antibody. (D) Western blot analyses of transformed E. coli lysates employing the anti-GFP antibody.