Expression of Cre recombinase from phINS-Cre plasmid.

(a) Schematic picture of phINS-Cre construct. (b) Immunofluorescence analysis of HIT-T15 and MB-231 cells transiently transfected with phINS-Cre. The red color indicates Cre recombinase expression. Scale bar: 10 µm. (c) Immunostaining of HIT-T15 cells transiently co-transfected with phINS-Cre and pEGFP-N1. The red color indicates Cre recombinase expression (Cy-3), and the green is a marker for co-transfection. (d) Immunostaining of stable HIT-T15 FACS-sorted GFP⁺ and mCherry⁺ cells with and without the primary anti-Cre antibody. The green colors in the top and middle panels were visualized as EGFP expression, and the red (Cy-3) as Cre recombinase expression. The stable FACS-sorted mCherry⁺ cells in the bottom panels did not express Cre recombinase (no green, Cy-2).