Experimental validation of two genes identified by ZODET.

A. PCR verification of the TFG-GPR128 translocation in individual B1 who was identified as an outlier for GPR128 using ZODET analysis. B. Expression profile for the GPR128 probe (ILMN_2125395) in controls and experimental subjects. Green arrow identifies an individual who carries the TFG-GPR128 translocation, demonstrates elevated GFP128 levels but was not identified by the ZODET analysis using the p<0.0001 and fold change >1.75 thresholds. C. Genomic DNA sequencing of the OAS1 gene revealed a splice site polymorphism A>G (rs10774671) in all individuals who were identified as outliers by the ZODET analysis. All six individuals were shown to be homozygous for the polymorphism. D. Expression profile for the OAS1 probe (ILMN_1658247) in controls and experimental subjects. Green arrow identifies an individual who carries the splice site polymorphism, demonstrates reduced OAS1 levels but was not identified by the ZODET analysis using the p<0.0001 and fold change >1.75 thresholds.