Fig_1.tif (3.11 MB)

Experimental set-up and global omics analyses.

figure

posted on 2015-06-03, 04:06 authored by Sebastian Oeder, Tamara Kanashova, Olli Sippula, Sean C. Sapcariu, Thorsten Streibel, Jose Manuel Arteaga-Salas, Johannes Passig, Marco Dilger, Hanns-Rudolf Paur, Christoph Schlager, Sonja Mülhopt, Silvia Diabaté, Carsten Weiss, Benjamin Stengel, Rom Rabe, Horst Harndorf, Tiina Torvela, Jorma K. Jokiniemi, Maija-Riitta Hirvonen, Carsten Schmidt-Weber, Claudia Traidl-Hoffmann, Kelly A. BéruBé, Anna J. Wlodarczyk, Zoë Prytherch, Bernhard Michalke, Tobias Krebs, André S. H. Prévôt, Michael Kelbg, Josef Tiggesbäumker, Erwin Karg, Gert Jakobi, Sorana Scholtes, Jürgen Schnelle-Kreis, Jutta Lintelmann, Georg Matuschek, Martin Sklorz, Sophie Klingbeil, Jürgen Orasche, Patrick Richthammer, Laarnie Müller, Michael Elsasser, Ahmed Reda, Thomas Gröger, Benedikt Weggler, Theo Schwemer, Hendryk Czech, Christopher P. Rüger, Gülcin Abbaszade, Christian Radischat, Karsten Hiller, Jeroen T. M. Buters, Gunnar Dittmar, Ralf Zimmermann

(A) An 80 KW common-rail-ship diesel engine was operated with heavy fuel oil (HFO) or refined diesel fuel (DF). The exhaust aerosols were diluted and cooled with clean air. On-line real-time mass spectrometry, particle-sizing, sensor IR-spectrometry and other techniques were used to characterise the chemical composition and physical properties of the particles and gas phase. Filter sampling of the particulate matter (PM) was performed to further characterise the PM composition. Lung cells were synchronously exposed at the air-liquid-interface (ALI) to aerosol or particle-filtered aerosol as a reference. The cellular responses were characterised in triplicate at the transcriptome (BEAS-2B), proteome and metabolome (A549) levels with stable isotope labelling (SILAC and ¹³C₆-glucose). (B) Heatmap showing the global regulation of the transcriptome, proteome and metabolome.