Exogenous recombinant heparanase (rHPSE) decreases nuclear syndecan-1 levels in a concentration-dependent manner.

Recombinant heparanase was added to CAG cells having very low levels of heparanase expression (shRNA knockdown cells). Nuclear and non-nuclear fractions were prepared and syndecan-1 levels analyzed by A) western blotting and B) ELISA. Grey bars = non-nuclear fraction; Black bars = nuclear fraction. Error bars represent standard error of the mean. *, P<0.01 vs. nuclear syndecan-1 in cells treated with 0 ng/ml rHPSE.