Figure_2.tif (4.36 MB)
Example of results of the semi-quantitative SLM drop assay showing various categories of mutator phenotype.
figure
posted on 2011-06-17, 01:17 authored by Malgorzata Alabrudzinska, Marek Skoneczny, Adrianna SkonecznaCell suspensions were serially diluted and spotted onto selection plate (with canavanine or 5′-FOA) and onto dilution control plate as described in Materials and Methods. WT – SLM level in parental strain, M - increased SLM phenotype, HM - high SLM phenotype, ρ− - increased SLM due to respiratory incompetence in WT ρ− strain, Rρ− – resistance to selection conditions acquired along with the loss of respiratory competence, M/GD - high SLM phenotype accompanied by decreased survival rate, seen also without selection, R - full resistance to selection conditions.