Enzymatic incorporation of LNA-T nucleotides by SuperScript® III Reverse Transcriptase.
figure
posted on 2012-04-25, 02:24 authored by Lucile Crouzier, Camille Dubois, Stacey L. Edwards, Lasse H. Lauridsen, Jesper Wengel, Rakesh N. Veedu(A) Primer and template sequences. (B) Gel electrophoresis of incorporation LNA-T, lane 1: Primer, 19 nt; lane 2: Positive control using all four natural dNTPs; lane 3: Negative control reaction with only three natural nucleotides (dCTP, dATP, dGTP); lane 4: Incorporation of LNA-T (dCTP, dATP, dGTP, LNA-TTP in the mixture). (C) Gel electrophoresis of incorporation LNA-T at 5–45 minute incubation time points, lane P1: Primer. LNA nucleotides are underlined and the incorporation sites in the template RNA are italicized.
