Embryonic lethality of mice homozygous for the Lipi-Usp25 deletion.
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posted on 2012-01-20, 00:23 authored by Anna M. Migdalska, Louise van der Weyden, Ozama Ismail, Jacqueline K. White, Sanger Mouse Genetics Project, Gabriela Sánchez-Andrade, Darren W. Logan, Mark J. Arends, David J. Adams(A) Genotyping of E10.5, E12.5, E14.5 embryos, and 3-week old mice from monosomic Ms1Dja intercrosses. Asterisk indicates P<0.01 (Chi-square test). (B) Photos of wildtype (+/+), monosomic (Ms1Dja/+) and nullisomic (Ms1Dja/Ms1Dja) embryos at E10.5. Longitudinal measurements are 4 mm, 4 mm and 2.7 mm for the wildtype, monosomic and nullisomic embryos, respectively. (C) Immunohistochemical analysis using a Cleaved Caspase-3 (Asp175) antibody showed markedly increased number of apoptotic cells (brown staining; highlighted by black arrows) in the neural tube of nullisomic (Ms1Dja/Ms1Dja) E10.5 embryos. Images are representative and taken at ×200 magnification.
