Effects on Lifespan in C. elegans of RNAi of nac-2, nac-3, and daf-2 (22 °C) Lifespan analysis of the mutants subjected to bacteria-mediated RNAi in nonpreinduced and preinduced conditions (see text for details). N2 (wild-type) worms fed with L440 plasmid was used as a vector control. Three independent replicates of the experiments are shown. Log-Rank test χ² and p-values:

A) L4440 versus nac-2 (χ² = 1.82, p = 0.1772), L4440 versus nac-3 (χ² = 4.01, p = 0.0452), and L4440 versus daf-2 (χ² = 137.99, p < 0.0001).

B) L4440 versus nac-2 (χ² = 1.02, p = 0.8923), L4440 versus nac-3 (χ² = 0.52, p = 0.4727), and L4440 versus daf-2 (χ² = 39.11, p <0.0001).

C) L4440 versus nac-2 (χ² = 3.82, p = 0.0507), L4440 versus nac-3 (χ² = 5.09, p = 0.024), and L4440 versus daf-2 (χ² = 65.76, p <0.0001).

D) The efficiency of the RNAi of nac-2 and nac-3 by the two methods of induction used for lifespan experiments (RT-PCR). On average, nac-2 was decreased by 85% with nonpreinduced method (npi) or by 62% with preinduced method. Nac-3 was decreased by 71% nonpreinduced or 30% preinduced. Each bar represents average (and standard error) from two measurements from independent RNA extractions.

npi, nonpreinduced; pi, preinduced.