Effects of oral LASW1 on T-cell proliferation.

Single splenocyte suspension was isolated from animals 14 days after the booster immunization, plated in triplicate in a 96-well plate and stimulated in vitro for 48 h with 146S antigen, ConA (positive control), BSA (irrelevant control), and medium (negative control). Proliferation was analyzed by the MTS assay and expressed as stimulation index.