Effect of baicalein on cisplatin-induced NF-κB related proteins.

Immunoblot analyses showing (A) nuclear translocation of NF-κB (p65), (B) phospho-IKKα/β, phospho-IκBα and IκBα expressions in kidneys. (C) NF-κB (p65)-DNA binding activity, where values are expressed as means ± SEM (n = 6). Immunoblots were representative of three independent experiments. Lamin B was used as internal control for nuclear fraction and β-actin was used as internal control for cytoplasmic and total protein fractions. Bar diagram showing densitometric analysis for relative expression of (D) nuclear NF-κB (p65)/cytoplasmic NF-κB (p65) ratio, phospho-IKKα/β-actin and phospho-IκBα/IκBα ratio. Values are the means ± SEM (n = 3). Where Control, group of animals treated with vehicle (2% gum acacia suspension, orally) daily for 15 consecutive days; Cisplatin, group of animals treated with 2% gum acacia suspension (orally) daily for 15 consecutive days and a single intraperitoneal (i.p) injection of cisplatin (20 mg/kg body weight, dissolved in normal saline) on 12^th day; Cis+Bai-50, group of animals treated with baicalein (50 mg/kg, orally) daily for 15 consecutive days and a single intraperitoneal (i.p) injection of cisplatin (20 mg/kg body weight, dissolved in normal saline) on 12^th day; ^#p< 0.05 and ^$p< 0.001 vs. vehicle control group, *p< 0.05, **p< 0.01 and ***p< 0.001 vs. cisplatin control group.