Effect of PCN treatment on energy expenditure of brown adipose tissue.

Six-week-old male AKR/J mice were fed with high-fat diet for two weeks with three injections per week of PCN (50 mg/kg) or DMSO (control). By the end of the feeding period, mice were exposed at 4°C and the rectal temperature measured at different times. A, time-dependent rectal temperature. For determination of the relative mRNA level of genes involved in thermogenesis, animals at the end of 7-week treatment were injected with PCN or carrier solution and sacrificed 4 h later. BAT tissues were harvested and total RNA was extracted. B. Relative mRNA levels of genes involved in thermogenesis. Each data point represents the average ± SD of 4 animals in each group. #P<0.05, ##P<0.01 compared to chow-DMSO group; *P<0.05, **P<0.01 compared to high-fat diet-DMSO group. Abbreviations: PGC-1, peroxisome proliferator-activated receptor gamma coactivator 1 gene; Dio2, Type II iodothyronine deiodinase gene; Cidea, cell death-inducing DNA fragmentation factor alpha-like effector A gene; and UCP, uncoupling protein gene.