Effect of 5′/3′YY1 mutations on the exon 1a promoter transcriptional activity.

HeLa and EA.hy926 cells were transfected with 500 ng of TSSC mutants described in Fig. 2B and Table 1. 5 ng of pRL-CMV was added as an internal control for each transfection, and relative luciferase activity is shown as the means based on the activity of +7/+266. Data represent mean ± S.E.M. (n = 4). *P<0.05 versus −106/+266.