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EZH2 expression in colon cancer cell lines.
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posted on 2011-07-13, 02:06 authored by Bettina Fussbroich, Nina Wagener, Stephan Macher-Goeppinger, Axel Benner, Maria Fälth, Holger Sültmann, Angela Holzer, Karin Hoppe-Seyler, Felix Hoppe-SeylerA Immunoblot analysis of EZH2 protein expression. Tubulin, loading control. B qRT-PCR analyses of EZH2 mRNA expression. Data are presented as fold differences in gene expression, normalized to a housekeeping gene index. Standard deviations from two reverse transcription replicates are indicated. C Modulation of EZH2 protein expression by RNAi. EZH2 expression was determined by immunoblot analysis 48 hours after transfection with EZH2-targeting siRNAs or control siRNAs, as indicated. siEZH2pool: pooled EZH2-targeting siRNAs. Tubulin, loading control.
