EMT-phenotype detection of spheroid/adherent cells.
figure
posted on 2013-09-09, 02:35 authored by Xiao-Yan Han, Bo Wei, Jia-Feng Fang, Shi Zhang, Fu-Cheng Zhang, Hai-Bo Zhang, Tian-Yun Lan, Hui-Qiong Lu, Hong-Bo WeiRepresentative photomicrographs depicting the higher expression of E-cadherin and the membrane localization of β-catenin in HCT116 and HT-29 adherent cells than the corresponding spheroid cells by immunofluorescence staining. And higher expression of Vimentin in spheroid cells was detected comparing to adherent cells.
