Figure_5.tif (846.22 kB)
EGF stimulation of Hedgehog-primed Daoy cells strongly upregulated canonical EGF target genes.
figure
posted on 2013-06-10, 01:31 authored by Frank Götschel, Daniela Berg, Wolfgang Gruber, Christian Bender, Markus Eberl, Myriam Friedel, Johanna Sonntag, Elena Rüngeler, Hendrik Hache, Christoph Wierling, Wilfried Nietfeld, Hans Lehrach, Annemarie Frischauf, Reinhard Schwartz-Albiez, Fritz Aberger, Ulrike KorfCells were treated with either control medium (purple), Shh-N conditioned medium (blue), EGF (red) or a combinatorial treatment with Shh-N medium and EGF (green). (A) Gene expression profiling data indicated upregulation of MMP7, VEGFA, and IL-8. (B) Taqman Real Time PCR confirmed upregulation of canonical EGF targets in Hedgehog-primed cells. (C) MMP7, VEGFA, and IL-8 protein levels were quantified by ELISA in cell culture supernatants at indicated time points. Average fold-changes or concentrations were obtained as mean of three independent biological experiments (+/−SEM).
History
Usage metrics
Categories
Keywords
Computational biologygenomicsGenome analysis toolstranscriptomesMolecular cell biologySignal transductionSignaling in cellular processesTranscriptional signalingSignaling in selected disciplinesOncogenic signalingproteomicsoncologyBasic cancer researchstimulationhedgehog-primeddaoycellsupregulatedcanonicalegf
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC