EGFR phosphorylation is amplified in the immobile state.

(A) Probability of colocalization between Cy3-SNAP-EGFR and EGFP-PTB. (B) Normalized intensity histograms of the Cy3-SNAP-EGFR intensity, for all particles (continuous lines) and for the particles that colocalized with EGFP-PTB (dashed lines) after 10 minutes of EGF stimulation. (C) Probability of colocalization between Cy3-SNAP-EGFR and EGF-Alexa488. (D) Fold-increase of the colocalization probability in the immobile versus the confined state, for EGFP-PTB colocalization (see A), and for EGF-Alexa488 colocalization (see C). A, B: n = 43 cells; C, D: n = 36 cells. ns: P > 0.05, **P < 0.01, ***P < 0.001, t test. Error bars denote SEM.