E6 reduces the luciferase activities of the full length and a series of deletion constructs of the human E-cadherin regulatory region, whilst reducing the occupancy of the transcriptional regulators within this region.

(a) The pGL3 Firefly luciferase constructs were transfected into HCT116 (black bars) and HCT116 E6 (white bars) cells and 48 h later luciferase activities were measured. Activity was normalized to pRL-SV40 Renilla luciferase activity. Results are from three independent experiments. (b) HCT116 and HCT116 E6 cells were subjected to ChIP analysis followed by PCR to determine occupancy of transcriptional regulators AML1, Sp1 and Slug within the E-cadherin regulatory region. (c) Three independent experiments were quantified (Image J, NIH) to measure the relative percentage of AML1, Slug or Sp1 bound to the E-cadherin promoter in E6 cells relative to control cells.