Down-regulation of RPH3AL mRNA and protein in breast cancers.

Analyses of mRNA and protein expression of RPH3AL(A) mRNA levels of RPH3AL down-regulated in breast cancers relative to their matching normal tissues. (B) Immunohistochemical staining of RPH3AL in breast cancer tissues. RPH3AL expression was seen predominantly in the cytoplasm of (i) lobular intraepithelial neoplasia (LIN, orange arrow) and adjacent normal cells (green arrow) (X100) (ii) ductal carcinoma in situ (DCIS, red arrow) and adjacent normal cells (green arrow) (X100) (iii) DCIS (red arrow) and invasive carcinoma (dark red) (X200). LIN and DCIS exhibit lower expression of RPH3AL relative to adjacent normal cells. Mild nuclear staining was also seen in a few cells of normal, LIN, DCIS, and invasive carcinoma.