Dot blot assay for co-expression of multiple MTases.
figure
posted on 2012-09-27, 02:16 authored by Guoqiang Zhang, Wenzhao Wang, Aihua Deng, Zhaopeng Sun, Yun Zhang, Yong Liang, Yongsheng Che, Tingyi WenSerial dilutions of DNA (450 ng–50 ng) from the E. coli EC135 strain harboring pM.Bam, pM.Bce or pM.Nham were tested, and the corresponding DNA from the native host of the MTases was used as positive controls. DNA of the E. coli EC135 strain harboring pWYE724 was used as a negative control. Antibodies against m6A, m4C and m5C were used in the upper, middle, and lower panels, respectively. All experiments were repeated at least three times, and representative results are shown.
