Percentage of discriminator “Hoshioki” workers that bit at the CHC-coating glass beads as surrogate ants are plotted against the ant equivalent amount of CHCs (total amount of components) derived from “Hoshioki”, “Shinkawa”, Hakkenzan”, and C. japonicus workers. The fitting curves were drawn by Igor pro ver.4.0, under the hypothesis that all the discriminators could show biting behavior toward infinitely large amounts of CHCs, regardless of their nests. The CHC amounts giving half the maximum response, which is defined as the mean threshold amounts of CHCs for inducing biting behavior, are indicated by arrowheads.