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Distribution of antigen presenting cells in peripheral blood during chronic HIV infection.

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posted on 2013-08-30, 02:21 authored by Lauren H. Nagy, Irina Grishina, Monica Macal, Lauren A. Hirao, William K. Hu, Sumathi Sankaran-Walters, Christopher A. Gaulke, Richard Pollard, Jennifer Brown, Maria Suni, Andreas J. Baumler, Smita Ghanekar, Maria L. Marco, Satya Dandekar

(A) Multicolor flow cytometric gating strategy for the identification of APC populations in peripheral blood. APCs (CD3-CD19-CD56-HLADR+CD11c+CD123-), monocytes (CD3-CD19-CD56-HLADR+CD123-CD11c+CD14+), and mDCs (CD3-CD19-CD56-HLADR+CD123-CD11c+CD14+) were detected following the exclusion of dead cells and CD3+, CD19+ and CD56+ cells. The HLA-DR-positive cells were gated for CD11c and CD123. Data were analyzed using FlowJo. (B) Percentages of circulating APCs (HIV− n = 37, HIV+ n = 94). (C) Monocyte percentage of the peripheral blood APC population. (D) Myeloid dendritic cell population of the peripheral blood APC population. (E) Plasma sCD14 concentrations as determined by ELISA (HIV− n = 25; HIV+ n = 66). Each dot represents an individual patient. In the HIV+ group, open circles represent therapy-naïve patients and closed circles represent patients on HAART. Bars indicate median value. P values determined using Mann Whitney U test. P values as indicated.