Distinct expression pattern of CBLN4 during embryonic development and subtle effects on axon pathfinding in CBLN4−/− mice.

A) Lac-Z reporter expression of CBLN4 in E11.5 CBLN4^+/− mice (top panels) showing distinctive expression in limbs including dorsal limb expression. Lac-Z reporter staining of CBLN4 in E13.5 CBLN4^+/− mice (lower panels) showing expression in the spinal cord motor column. B) commissural axons of E11.5 spinal cord visualized with TAG-1 immunostaining in combinations of CBLN4 and Netrin-1 genotypes. C) Representative images and quantification of wandering axons (white arrows) in the brachial plexus of E11.5 CBLN4^−/− mice. Tukey contrasts; **, P<0.01; ***, P<0.001; n is left and right forelimb nested in mouse; n = 4 CBLN4^+/+; n = 5 CBLN4^+/−; n = 6 CBLN4^−/−.