Figure_3.tif (3.46 MB)

Differential response of WT and transgenic rice lines under MV treatment.

figure

posted on 2013-12-03, 03:55 authored by Chunchao Wang, Qiang Wei, Kang Zhang, Ling Wang, Fengxia Liu, Linna Zhao, Yuanjun Tan, Chao Di, Hong Yan, Jingjuan Yu, Chuanqing Sun, Wenqiong J. Chen, Wenying Xu, Zhen Su

A. Four-day-old seedlings treated with 10 μM MV (MV) and 10 μM MV plus 10 mg/L GSH (MV+GSH) for 3 d, water as mock. A1 and A2 are Ubi::OsSPX1-antisense transgenic lines (on the left), S1 and S2 are Ubi::OsSPX1-sense lines (on the right).

B. The chlorophyll content of seedlings under MV treatment (blue bars for Ubi::OsSPX1-antisense and red bars for Ubi::OsSPX1-sense).

C. DAB staining of the seedling leaves treated with 10 μM MV, water as mock. A1 and A2 are Ubi::OsSPX1-antisense transgenic lines (on the left), S1 and S2 are Ubi::OsSPX1-sense lines (on the right).

D. The survival rate of WT and transgenic plants treated with 10 μM MV, with/without GSH.

E. H₂O₂ content of the leaves of WT and transgenic plants under MV treatment (blue bars for Ubi::OsSPX1-antisense and red bars for Ubi::OsSPX1-sense).

F. Peroxidase activity in WT and transgenic rice plants under MV treatment (blue bars for Ubi::OsSPX1-antisense and red bars for Ubi::OsSPX1-sense).

** represent significant differences (p < 0.01) between transgenic and WT rice plants according to Student’s t-test.