Differential response of WT and transgenic rice lines under MV treatment.

A. Four-day-old seedlings treated with 10 μM MV (MV) and 10 μM MV plus 10 mg/L GSH (MV+GSH) for 3 d, water as mock. A1 and A2 are Ubi::OsSPX1-antisense transgenic lines (on the left), S1 and S2 are Ubi::OsSPX1-sense lines (on the right).

B. The chlorophyll content of seedlings under MV treatment (blue bars for Ubi::OsSPX1-antisense and red bars for Ubi::OsSPX1-sense).

C. DAB staining of the seedling leaves treated with 10 μM MV, water as mock. A1 and A2 are Ubi::OsSPX1-antisense transgenic lines (on the left), S1 and S2 are Ubi::OsSPX1-sense lines (on the right).

D. The survival rate of WT and transgenic plants treated with 10 μM MV, with/without GSH.

E. H2O2 content of the leaves of WT and transgenic plants under MV treatment (blue bars for Ubi::OsSPX1-antisense and red bars for Ubi::OsSPX1-sense).

F. Peroxidase activity in WT and transgenic rice plants under MV treatment (blue bars for Ubi::OsSPX1-antisense and red bars for Ubi::OsSPX1-sense).

** represent significant differences (p < 0.01) between transgenic and WT rice plants according to Student’s t-test.

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