Diagram of digital flow control box with flow controllers.

A diagram showing the set up of the digital control box and gas flow controllers. Inset diagrams the principle of the flow controls as adapted from the manual. Individual parts are detailed in Table 1 in addition to as follows: A: Model 954 Digital Flow Box, B: 840L Mass Flow Controller –10 SLPM (N₂) capacity, C: 840L Mass Flow Controller –1000 SCCM (CO₂) capacity, D: 840L Mass Flow Controller –1000 SCCM (O₂) capacity, E: 840-CDCL (15 ft) Cable, F: Compression fitting Union Cross, G: Compression threaded adapter, H: Barbed fittings, I: Copper tubing, J: Brass tubing, K: Clear PVC tubing, L: Pall Acro 0.2 µm PTFE vent filter, M: Modular Incubator Chamber, N: N₂/CO₂ Gas regulator, O: O₂ Gas regulator, P: (Optional) 840L Mass Flow Controller –1000 SCCM (Air). Gas flow rates shown for B, C, and D correspond to a total flow rate of 10 SLPM at the appropriate gas percentages for parasite culture (94% N₂, 5% CO₂, 1% O₂).