Determination of molecular mass by (A) gel filtration (Kav is function of the elution volume of a molecule and indicates the ratio between the elution volume of a given molecule and the total available volume of the column), and native PAGE showing single band of purified enzyme.

(B) SDS PAGE of protein eluted from 5 step purification of cytosolic (ARG I) of H. fossilis liver. Lanes-1, crude cytosolic fraction; 2, supernatant of heat treatment; 3, ion exchange chromatography; 4, gel filtration chromatography; 5, affinity chromatography; 6, standard protein marker. 24 kDa and 48 kDa represent the molecular weight of monomer and dimer of purified ARG I (arginine sepharose affinity column eluted protein) on SDS-PAGE. (Representative of three independent purification experiments).