Detection of apoptosis in astrocytes that were exposed to oxygen-glucose deprivation for 24 hours.

Apoptosis was induced in cultured astrocytes for 24 hours in an OGD chamber detected using an Annexin-V assay. Astrocytes that were exposed OGD-alone displayed a higher incidence of apoptosis as indicated by green Annexin-V staining along the membranes of the astrocytes. Astrocytes that were exposed to one of relaxin-2, relaxin-3 or R3/I5 and OGD showed a lower incidence of apoptosis.