Detection of CD11b-positive and CD3-expressing cells in pancreatic tissue.

All samples are from 12-month-old B6-mtFVB mice but are representative for animals of both strains. Pancreatic sections of mice at the indicated time points after the start of cerulein treatment were stained with anti-CD11b and anti-CD3 antibodies, respectively. Upper row: large numbers of scattered CD11b-positive cells at 24 h (left), only background staining on day 7 (right). Lower row: 24 h (left) – faint background staining only in a selected region without autoimmune-like pancreatic lesions; day 7 (right): focal infiltrate of inflammatory cells, many of them CD3-positive; background staining of the surrounding pancreatic tissue.