A) Neuronal differentiation of 201B7-derived hNCCs. Cells were stained with an antibody against peripherin (red) and Tuj-1 (green). B) The glial differentiation of 201B7-derived hNCCs. Cells were stained with an antibody against GFAP. Scale bar, 50 µm. C) Melanocyte differentiation of 201B7-derived hNCCs. The mRNA expression levels of MITF and c-KIT genes were shown as a relative value using the value in 201B7-derived hNCCs and 201B7 as 1.0, respectively. Average ± SD. N = 3, biological triplicates.