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Derivation and Characterization of FXS Induced Pluripotent Stem Cells.

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posted on 12.10.2011 by Steven D. Sheridan, Kraig M. Theriault, Surya A. Reis, Fen Zhou, Jon M. Madison, Laurence Daheron, Jeanne F. Loring, Stephen J. Haggarty

(A) Alkaline phosphatase enzymatic and pluripotent marker (OCT4, NANOG and SSEA-4) immunocytochemical analysis of FXS patient-derived iPSC clones. (B) Endogenous OCT4, NANOG and REX1 pluripotency-associated transcript expression as analyzed by RT-PCR in indicated iPSC lines and fibroblasts (NTC - non-template containing control). (C) Bisulphite pyrosequencing analysis of the endogenous OCT4/POU5F1 promoter in indicated lines (open circles, unmethylated (<50%) CpGs; black circles, methylated CpGs). (D) Embryoid body pathological evaluation of H&E stained sections (clone 848-iPS1 shown) indicating representative ectoderm (neural epithelium, left), endoderm (respiratory epithelium, center) and mesoderm (connective tissue, right) germ layers.