Depicted is a vector map of shuttle vector pBSU101 that was used in this study to label different gram-positive bacteria with EGFP.

The multiple cloning site (MCS), the different origins of replication (for use in gram-positive hosts, oriR pAMß1, and gram-negative bacteria, oriR pUC) and the spectinomycin (spc) resistance gene are indicated. The plasmid is a derivative of pAT28. Expression of EGFP in pBSU101 is controlled via the promoter region of the CAMP-factor gene cfb of Streptococcus agalactiae. The plasmid carries the promoter region and the ATG startcodon of cfb, resulting in a translational fusion of the first 7 amino acids of CFB to the N-terminus of EGFP as depicted. Between the CFB fragment and the start of EGFP a small spacer of 8 amino acids is found that does not appear to disturb efficient EGFP expression.