Deletion of YIH1 leads to the accumulation of cells in the G2/M phases of the cell cycle.

(A) Representative images of cells grown asynchronously to log phase in YPD medium, spheroplasted with zymolyase, permeabilized, fixed and stained with DAPI to visualize the nuclear DNA (left panels) and with anti-tubulin antibodies followed by Alexa-488 anti-mouse IgG to visualize spindles (middle panels). (B) Cells prepared as in A were counted and the percentage of cells with medium sized-buds and short spindles and the localization of the nucleus as indicated (left), or with large buds and elongated spindles (right), were determined. (C) Representative images of live exponentially growing yeast cells stained with DAPI. (D) Quantification of the percentage of cells in G1 (unbudded), S (cells with small buds) and G2/M (cells with medium to large buds) determined from (C). Over 450 cells were analyzed per experiment. Data represent the mean ± S.E. as error bars of three independent experiments for B and D. * p< 0.05; (Student´s t test).