Degenerate PCR and sequencing primers designed using CODEHOP software.

Nucleotides comprising the 3′ degenerate tail are in small letters. Nucleotides forming the 5′ non-degenerate clamp are in large letters. Amino acids comprising the degenerate tail are highlighted. Primers with the degenerate tail highlighted in black were used for PCR only. Primers with the degenerate tail highlighted in blue were used for both PCR and sequencing. The primer with the degenerate tail highlighted in red was an internal degenerate sequencing primer required for clean sequence.