Definitive and extraembryonic endoderm differentiated from G6pdΔ ES cells.

(A) Immunofluorescent staining of the differentiated mouse G6pdΔ ES cells for Sox17 (red) and nuclei (blue) at 8 days of neural differentiation. PH, phase contrast images. Scale bars, 25 µm. (B) RT-PCR analysis of the endodermal markers GATA4 and extraembyonic endodermal markers Sox7 during differentiation. Lane C, positive control, RNA isolated from embryos and yolk sacs at 9,5 dpc. Amplified HPRT is shown as a positive control. (C) qRT-PCR for Pdx1 in wt and G6pdΔ ES cells at 13 days after treatment with Indolactam V from day 8 during differentiation. Values are means ± SD (n = 2). *P<0.05; **P<0.01; ***P<0.001.