DNR induced autophagy in K562 cells.
A. K562 cells were incubated with varying concentrations of DNR for 24 hours or incubated with 1.25 µg/ml DNR for appropriate intervals. The switch of LC3-I to LC3-II was detected by immunoblotting. B. Immunoblotting for Beclin-1 using lysates from K562 treated with 1.25 µg/ml DNR for appropriate intervals. C. MDC staining of K562 treated with DNR. Cells treated with 1.25 µg/ml DNR in the presence or absence of 5 µM CQ for 24 hours were stained with MDC. D. K562 cells were treated with 1.25 µg/ml DNR for 24 hours, and stained by indrect immunofluorescence as described in Marterial & Methods. The distribution of endogenous LC3 was monitored at confocal microscope.