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DNA doubling efficiency.

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posted on 2016-01-18, 15:28 authored by Alexa J. Bracht, Emily S. O’Hearn, Andrew W. Fabian, Roger W. Barrette, Abu Sayed

Linear amplification of circular plasmid DNA was between copy numbers of 4 x 100 and 4 x 106, and generated a standard curve with calculated efficiency of 86% from y-slope (-3.72) with correlation coefficient (R2) of 0.9993.

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