Cytokines response 1 month after challenge.

Immune response in the lymph nodes of hamsters immunized with LdCen^-/- and LJM19 by qPCR (A to E). The mRNA expression of IFN-γ (A), iNOS (B), IL-12(C), IL-4 (D), and IL-10 (E) was evaluated. The data were normalized to β-actin expression. Fold increase of the cytokines compared to BSA (G4) group (F). Immune response in the spleens of hamsters immunized with LdCen^-/- and LJM19 by qPCR (G to K). The mRNA expression of IFN-γ (G), iNOS (H), IL-12(I), IL-4 (J), and IL-10 (K) was evaluated. The data were normalized to β-actin expression. Fold increase of the cytokines compared to BSA (G4) group (L). Immune response in the livers of hamsters immunized with LdCen^-/- and LJM19 by qPCR (M to Q). The mRNA expression of IFN-γ (M), iNOS (N), IL-12(O), IL-4 (P), and IL-10 (Q) was evaluated. The data were normalized to β-actin expression. Fold increase of the cytokines compared to BSA (G4) group (R). Statistical differences (p<0.05) are indicated in letters (a: G1, b: G2 and c: G3).